Survival Strategies Research Articles

Characteristics and immune functions of the endogenous CRISPR-Cas systems in myxobacteria.

Serving as an adaptive immune system, clustered regularly interspaced short palindromic repeats and their associated proteins (CRISPR-Cas) empower prokaryotes to fend off the intrusion of external genetic materials. Myxobacteria are a collective of swarming Gram-stain-negative predatory bacteria distinguished by intricate multicellular social behavior. An in-depth analysis of their intrinsic CRISPR-Cas systems is beneficial for our understanding of the survival strategies employed by host cells within their environmental niches. Moreover, the experimental findings presented in this study not only suggest the robust immune functions of CRISPR-Cas in myxobacteria but also their potential applications.

Dynamics and drivers of fungal communities in a multipartite ant-plant association

BackgroundFungi and ants belong to the most important organisms in terrestrial ecosystems on Earth. In nutrient-poor niches of tropical rainforests, they have developed steady ecological relationships as a successful survival strategy. In tropical ant-plant mutualisms worldwide, where resident ants provide the host plants with defense and nutrients in exchange for shelter and food, fungi are regularly found in the ant nesting space, inhabiting ant-made dark-colored piles (“patches”). Unlike the extensively investigated fungus-growing insects, where the fungi serve as the primary food source, the purpose of this ant-fungi association is less clear. To decipher the roles of fungi in these structures within ant nests, it is crucial to first understand the dynamics and drivers that influence fungal patch communities during ant colony development.ResultsIn this study, we investigated how the ant colony age and the ant-plant species affect the fungal community in the patches. As model we selected one of the most common mutualisms in the Tropics of America, the Azteca-Cecropia complex. By amplicon sequencing of the internal transcribed spacer 2 (ITS2) region, we analyzed the patch fungal communities of 93 Azteca spp. colonies inhabiting Cecropia spp. trees. Our study demonstrates that the fungal diversity in patches increases as the ant colony grows and that a change in the prevalent fungal taxa occurs between initial and established patches. In addition, the ant species significantly influences the composition of the fungal community in established ant colonies, rather than the host plant species.ConclusionsThe fungal patch communities become more complex as the ant colony develops, due to an acquisition of fungi from the environment and a substrate diversification. Our results suggest a successional progression of the fungal communities in the patches during ant colony growth and place the ant colony as the main driver shaping such communities. The findings of this study demonstrate the unexpectedly complex nature of ant-plant mutualisms in tropical regions at a micro scale.

Women’s Homelessness and the Justice System: A Study of Desistance and Social (Re)integration Among Canadian Women Who Used or Did Not Use Criminal Activities to Survive

Women experiencing homelessness (WEH) are a marginalized group who often lack support services, leading them to adopt alternative survival strategies that leave them at risk of being victimized and becoming involved with the justice-system. To better understand this problem, we analyzed the adaptive strategies Canadian WEH use to survive. Comparing the life histories of WEH who turned to criminal activities ( n = 4) with WEH who demonstrated prosocial resilience ( n = 4) makes it possible to identify protective and risk factors for criminal behavior and to propose adaptive strategies that can be used to support these women to adopt a prosocial lifestyle. The results provide a framework for understanding the needs of WEH, filling a gap that results from the focus on men needs in most scientific literature and by many resources, and suggesting that meeting those needs may reduce the likelihood that WEH will become involved with the justice-system.

Antarctica's hidden mycoviral treasures in fungi isolated from mosses: A first genomic approach.

This study investigates the presence of mycoviruses in Antarctic fungi and elucidates their evolutionary relationships. To achieve this, we aligned mycoviral gene sequences with genomes of previously sequenced Antarctic endophytic fungi, made available by our research group and accessible via Joint Genome Institute. Our findings reveal that the most prevalent genetic regions in all endophytic fungi are homologous to Partitiviruses, Baculoviridae, and Phycodnaviridae. These regions display evidence of positive selection pressure, suggesting genetic diversity and the accumulation of nonsynonymous mutations. This phenomenon implies a crucial role for these regions in the adaptation and survival of these fungi in the challenging Antarctic ecosystems. The presence of mycoviruses in Antarctic endophytic fungi may indicate shared survival strategies between the virus and its host, shedding light on their evolutionary dynamics. This study underscores the significance of exploring mycoviruses within endophytic fungi and their contributions to genetic diversity. Future research avenues could delve into the functional implications of these conserved mycoviral genetic regions in Antarctic endophytic fungi, providing a comprehensive understanding of this intriguing association and genomic retention of viral region in fungi.

Microscopic and molecular genetic analyses of morphological development of the actinomycete Actinoplanes missouriensis.

The survival strategy of members of the bacterial genus Actinoplanes is fascinating from morphological and evolutionary perspectives. A brief motile phase is incorporated in the filamentous and resting stages of the life cycle of Actinoplanes missouriensis. Spores either lie dormant or swim under different external conditions. This review presents microscopic observations and molecular genetic analyses of A. missouriensis morphological development. Selected examples of the characterization of developmental genes and their products are also introduced.

An antifungal compound secreted by termite workers, phenylacetic acid, inhibits the growth of both termite egg-mimicking fungus and entomopathogenic fungi

AbstractSocial insects live in closely related family groups but face risks of intrusion and infection by pathogenic and parasitic microbes. To cope with the microbes invading their nests and feeding sites, social insects produce various types of antimicrobial substances. Subterranean termites occupy microbe-rich decaying wood and soil at high density, expanding their nest area by exploring and feeding on wood outward from the royal chamber (room for kings and queens). Although antimicrobial agents have been identified in many termite species, few studies have investigated those used by foraging workers in decaying wood under development, which is richer in microbes than the well-sterilized royal chamber and its surroundings. Here, we report that phenylacetic acid, an antifungal aromatic compound, is secreted by foraging workers of the Japanese subterranean termite Reticulitermes speratus. The compound was detected by gas chromatography–mass spectrometry analysis of ethyl acetate extracts of shelter papers infested with the workers, and antimicrobial tests demonstrated that it inhibits the germination and/or mycelial growth of the entomopathogenic fungi (Metarhizium anisopliae and Beauveria bassiana) and the termite egg-mimicking fungus Athelia termitophila. Our study provides new insights into the antimicrobial defense mechanisms of termites, including by combining different types of antimicrobial substances secreted by different castes, and thus the survival strategy of entomopathogenic and parasitic fungi in termite nests.

Integrated metabolomic and metagenomic strategies shed light on interactions among planting environments, rhizosphere microbiota, and metabolites of tobacco in Yunnan, China

Changes in climatic factors and rhizosphere microbiota led plants to adjust their metabolic strategies for survival under adverse environmental conditions. Changes in plant metabolites can mediate crop growth and development and interact with rhizosphere microbiota of the plant rhizosphere. To understand the interactions among environmental factors, rhizosphere microbiota, and metabolites of tobacco, a study was conducted by using integrated metagenomic and metabolomic strategies at four typical representative tobacco planting sites in Yunnan, China. The results showed that the agronomical and biochemical traits were significantly affected by temperature, precipitation (PREP), soil pH, and altitude. Correlation analyses revealed a significant positive correlation of temperature with length, width, and area of the leaf, while PREP correlated with plant height and effective leaf numbers. Furthermore, total sugar and reducing sugar contents of baked leaves were significantly higher, while the total nitrogen and total alkaloid levels were lower in tobacco leaves at site with low PREP. A total of 770 metabolites were detected with the highest number of different abundant metabolites (DMs) at Chuxiong (CX) with low PREP as compared to the other three sites, in which secondary metabolites were more abundant in both leaves and roots of tobacco. A total of 8,479 species, belonging to 2,094 genera with 420 individual bins (including 13 higher-quality bins) harboring 851,209 CDSs were detected. The phyla levels of microorganisms such as Euryarchaeota, Myxococcota, and Deinococcota were significantly enriched at the CX site, while Pseudomonadota was enriched at the high-temperature site with good PREP. The correlation analyses showed that the metabolic compounds in low-PREP site samples were positively correlated with Diaminobutyricimonas, Nissabacter, Alloactinosynnema, and Catellatospora and negatively correlated with Amniculibacterium, Nordella, Noviherbaspirillum, and Limnobacter, suggesting that the recruitment of Diaminobutyricimonas, Nissabacter, Alloactinosynnema, and Catellatospora in the rhizosphere induces the production and accumulation of secondary metabolites (SMs) (e.g., nitrogen compounds, terpenoids, and phenolics) for increasing drought tolerance with an unknown mechanism. The results of this study may promote the production and application of microbial fertilizers and agents such as Diaminobutyricimonas and Alloactinosynnema to assemble synthetic microbiota community or using their gene resources for better cultivation of tobacco as well as other crops in drought environments.

The bubble theory: exploring the transition from first replicators to cells and viruses in a landscape-based scenario

AbstractThis study proposes a landscape-based scenario for the origin of viruses and cells, focusing on the adaptability of preexisting replicons from the RNP (ribonucleoprotein) world. The scenario postulates that life emerged in a subterranean “warm little pond” where organic matter accumulated, resulting in a prebiotic soup rich in nucleotides, amino acids, and lipids, which served as nutrients for the first self-replicating entities. Over time, the RNA world, followed by the RNP world, came into existence. Replicators/replicons, along with the nutritious soup from the pond, were washed out into the river and diluted. Lipid bubbles, enclosing organic matter, provided the last suitable environment for replicons to replicate. Two survival strategies emerged under these conditions: cell-like structures that obtained nutrients by merging with new bubbles, and virus-like entities that developed various techniques to transmit themselves to fresh bubbles. The presented hypothesis provides the possibility for the common origin of cells and viruses on rocky worlds hosting liquid water, like Earth.

Exploring the Scientific Mechanism of Tree Structure Network based on LiDAR Point Cloud Data

Abstract. To explore how trees optimize their structure, we developed a method based on Pareto optimality theory. This method consists of the following operations. Firstly, we utilize Quantitative Structure Models for Single Trees from Laser Scanner Data (TreeQSM) to extract tree structures from point clouds acquired through Light Detection and Ranging (LiDAR). Subsequently, we utilize a graph-theoretical model to characterize the natural tree structure networks and implement a greedy algorithm to generate Pareto optimal tree structure networks. Finally, based on the Pareto optimality theory, we explore whether tree structures are multi-objective optimized. This paper demonstrates that tree structures lie along the Pareto front between minimizing "transport distance" and minimizing "total length". The growth pattern of trees, which produces multi-objective optimized structures, is likely an intrinsic mechanism in the generation of tree structure networks. The location of tree structures along the Pareto front varies under different environmental conditions, reflecting their diverse survival strategies.

Starostniki na podeželju v 19. stoletju

Based on individual case studies and employing discourse analysis in Carniola, this study endeavours to transcend the existing theories that predominantly place the rural elderly within multigenerational familial structures. By delving into the intricacies of property transfer, the employment possibilities of individuals lacking assets, marital strategies, obstacles and dynamics, and the provision of care for elderly family members in rural areas, a nuanced understanding emerges. The present research reveals diverse strategies, identities, and configurations in the lifestyles and livelihoods of the rural elderly, which extend beyond conventional family-centric perspectives. By emphasising the intricate interplay of property dynamics, survival strategies, and communal solidarity, this article sheds light on the multifaceted life of the elderly in the countryside.

PSPC1 Binds to HCV IRES and Prevents Ribosomal Protein S5 Binding, Inhibiting Viral RNA Translation

Hepatitis C virus (HCV) infects the human liver, and its chronic infection is one of the major causes of Hepatocellular carcinoma. Translation of HCV RNA is mediated by an Internal Ribosome Entry Site (IRES) element located in the 5’UTR of viral RNA. Several RNA Binding proteins of the host interact with the HCV IRES and modulate its function. Here, we demonstrate that PSPC1 (Paraspeckle Component 1), an essential paraspeckle component, upon HCV infection is relocalized and interacts with HCV IRES to prevent viral RNA translation. Competition UV-crosslinking experiments showed that PSPC1 interacts explicitly with the SLIV region of the HCV IRES, which is known to play a vital role in ribosomal loading to the HCV IRES via interaction with Ribosomal protein S5 (RPS5). Partial silencing of PSPC1 increased viral RNA translation and, consequently, HCV replication, suggesting a negative regulation by PSPC1. Interestingly, the silencing of PSPC1 protein leads to an increased interaction of RPS5 at the SLIV region, leading to an overall increase in the viral RNA in polysomes. Overall, our results showed how the host counters viral infection by relocalizing nuclear protein to the cytoplasm as a survival strategy.

How are Trypanosoma brucei receptors protected from host antibody-mediated attack?

Trypanosoma brucei is the causal agent of African Trypanosomiasis in humans and other animals. It maintains a long-term infection through an antigenic variation based population survival strategy. To proliferate in a mammal, T. brucei acquires iron and haem through the receptor mediated uptake of host transferrin and haptoglobin-hemoglobin respectively. The receptors are exposed to host antibodies but this does not lead to clearance of the infection. Here we discuss how the trypanosome avoids this fate in the context of recent findings on the structure and cell biology of the receptors.

Food Sales Activities Associated to Family Survival Strategies During Covid-19 Lockdown in Brazzaville, Congo

The COVID-19 pandemic has a negative impact on people's living conditions, particularly in sub-Saharan Africa in vulnerable and low-income families. The aim of this work is to analyze the socio-demographic profile of people who engaged in food sales activities during the COVID-19 lockdown in Brazzaville, as well as their coping and survival strategies. The study, which used a mixed (qualitative and quantitative) approach, included 716 people (241 men and 475 women) aged 18 to 72 years residing in four communes in the southern zone of Brazzaville, Republic of Congo. The main study inclusion criterion was the practice of selling food during COVID-19 lockdown. However, we did not include people whose profession had been selling these products prior to the health crisis. The qualitative data collected during the interviews concerned information on vendors, sales locations, coping strategies used to find the products sold, and sales prices. As for the qualitative data assessed using a food questionnaire, they were summarized in terms of the quality and quantity of food consumed, which made it possible to assess daily energy intake and therefore the level of food security. The results showed that the sellers, with an average age of 42.7, had a relatively low level of education and lived in a medium-sized family (8). Of these, 21.8% were food insecure. Foodstuffs sold were dominated by miscellaneous products (48.2%), street foods (26.7%), ready-made meals (22.3%), cassava flour (17.4%) and fruit and vegetables (7.5%). Salted fish, smoked fish and frozen (imported) meat products were also sold. As far as prepared dishes were concerned, grilled meats of various kinds were the most popular. Sales outlets included state markets, neighborhood markets, storefronts and street markets for auctions. The use of rickshaws and wheelbarrows by a few money-seeking youngsters enabled the products purchased to be transported from the places of purchase to the various markets or sales sites. In short, given the absence of an effective distribution and marketing policy in the food system in times of crisis in the Congo, our results call into question the resilience of vulnerable populations in the face of the country's sociosanitary crises.

Integrating multi-platform assembly to recover MAGs from hot spring biofilms: insights into microbial diversity, biofilm formation, and carbohydrate degradation

BackgroundHot spring biofilms provide a window into the survival strategies of microbial communities in extreme environments and offer potential for biotechnological applications. This study focused on green and brown biofilms thriving on submerged plant litter within the Sungai Klah hot spring in Malaysia, characterised by temperatures of 58–74 °C. Using Illumina shotgun metagenomics and Nanopore ligation sequencing, we investigated the microbial diversity and functional potential of metagenome-assembled genomes (MAGs) with specific focus on biofilm formation, heat stress response, and carbohydrate catabolism.ResultsLeveraging the power of both Illumina short-reads and Nanopore long-reads, we employed an Illumina-Nanopore hybrid assembly approach to construct MAGs with enhanced quality. The dereplication process, facilitated by the dRep tool, validated the efficiency of the hybrid assembly, yielding MAGs that reflected the intricate microbial diversity of these extreme ecosystems. The comprehensive analysis of these MAGs uncovered intriguing insights into the survival strategies of thermophilic taxa in the hot spring biofilms. Moreover, we examined the plant litter degradation potential within the biofilms, shedding light on the participation of diverse microbial taxa in the breakdown of starch, cellulose, and hemicellulose. We highlight that Chloroflexota and Armatimonadota MAGs exhibited a wide array of glycosyl hydrolases targeting various carbohydrate substrates, underscoring their metabolic versatility in utilisation of carbohydrates at elevated temperatures.ConclusionsThis study advances understanding of microbial ecology on plant litter under elevated temperature by revealing the functional adaptation of MAGs from hot spring biofilms. In addition, our findings highlight potential for biotechnology application through identification of thermophilic lignocellulose-degrading enzymes. By demonstrating the efficiency of hybrid assembly utilising Illumina-Nanopore reads, we highlight the value of combining multiple sequencing methods for a more thorough exploration of complex microbial communities.

Which contributes more in predictor of desert shrubs traits: Intra-annual or inter-annual precipitation change?

Considering the implications of future climatic shifts, mid-latitude desert ecosystems will experience pronounced changes in precipitation dynamics, as evidenced by the elevated inter-annual variability and escalated annual incidence of extreme rainfall events. Despite considerable research on vegetation response to intricate precipitation patterns at the community level, the physiological adaptations of individual plants within these assemblages are not well defined. This investigation employed simulations of diverse precipitation scenarios, utilising varying precipitation amounts (to simulate inter-annual variability) and intervals (to simulate intra-annual variability). A field-manipulated experiment was conducted to assess the responses of the leaf and stem hydraulic functional traits of Artemisia ordosica under different precipitation regimes. The results demonstrated that 1) the magnitude of inter-annual precipitation, rather than the intra-annual redistribution of precipitation, dominated the variability in the functional traits of A. ordosica, with diminished inter-annual precipitation inducing an array of functional trait alterations (e.g., specific leaf area, stomatal conductance, water use efficiency, and conduit diameter) to enhance water-use safety. Reallocating precipitation to low-frequency but high-intensity events during years with less inter-annual precipitation was more advantageous for the survival of A. ordosica. 2) A reduction in annual precipitation fostered a correlation between stem and leaf functional traits in A. ordosica (e.g., hydraulic conductance with photosynthetic rate, stomatal conductance, conduit diameter with transpiration rate, and water use efficiency), suggesting that under decreased precipitation, A. ordosica strengthens the coupling between hydraulics and photosynthesis to prevent hydraulic failure, demonstrating a safer survival strategy. Overall, the impact of global changes in precipitation patterns on the individual survival of A. ordosica primarily depended on the variation in inter-annual precipitation rather than the within-year redistribution. This study enhanced our understanding of the adaptability of dominant shrub species within desert shrub communities to altered precipitation patterns under climate change.

Morphological and physiological features in small ruminants: an adaptation strategy for survival under changing climatic conditions.

Climate change due to natural human activity is a significant global phenomenon affecting the sustainability of most countries' livestock industries. Climate change factors such as ambient temperature, relative humidity, direct and indirect sun radiation, and wind have significant consequences on feed, water, pasture availability, and the re-emergence of diseases in livestock. All these variables have a considerable impact on livestock production and welfare. However, animals' ability to respond and adapt to changes in climate differs within species and breeds. Comparatively, small ruminants are more adaptive to the adverse effects of climate change than large ruminants in terms of reproduction performance, survival, production yield, and resistance to re-emerging diseases. This is mainly due to their morphological features against harsh climate effects. Tropical breeds are more adaptive to the adverse effects of climate change than small temperate ruminants. However, the difference in morphological characteristics towards adaptation to the impact of climate change will guide the development of suitable policies on the selection of breeding stock suitable for different regions in the world. The choice of breeds based on morphological features and traits is an essential strategy in mitigating and minimizing the effects of climate change on small ruminants' production and welfare. This review highlights the adaptive morphological features within and among breeds of small ruminants toward adaptation to climate change.

Collective Care Towards Homeostasis in the Collective Body

This article explores how rest as resistance pushes back against self-exploitation to introduce the concept of hegemony as organic matter. The author proposes we look at present and historical hegemonies as one unified growing organism, a collective body we ourselves are a part of, and is in a state of imbalance. Homeostasis is introduced as a concept that connects care, rest and collaboration as critical elements to bring about equilibrium. The author presents their degree project: a collaboration born as a survival strategy for two exhausted and almost burnt-out students. The article concludes with a call to care. 

Escherichia coli cells evade inducible parE toxin expression by reducing plasmid copy number.

This research has increased our understanding of how bacteria respond to the pressure from plasmid-borne toxic genes, such as those found in toxin-antitoxin systems. Surprisingly, we found that bacteria survived toxic ParE protein expression by reducing the number of these plasmids in the cells. This discovery reveals another way in which bacteria can balance toxin expression with antibiotic selection to attenuate the effects of deleterious genes. This insight is not only valuable for understanding bacterial survival strategies but may also influence the development of better tools in biotechnology, where plasmids are often used to study the functional roles of genes.

Proton-pumping rhodopsins promote the growth and survival of phytoplankton in a highly variable ocean.

Proton-pumping rhodopsins (PPRs) utilize sunlight to produce cellular energy. They are widely distributed in marine phytoplankton and were recently shown to occur in the vacuolar membrane of a marine diatom, making the vacuole a second light transducing organelle. Of course, the first, the chloroplast, is where photosynthesis occurs. However, the two light-driven sources of cellular energy are quite different and, in many ways, complement one another. Photosynthesis works best at low to intermediate light intensities, and is inhibited at high light, while PPR is predicted to work best at high light intensities. And photosynthetic rates decrease with decreasing temperature and are subject to iron limitation, while PPR photochemistry is not directly limited by iron, and is unaffected by temperature. Thus, the two phototrophic systems are favored under different sets of conditions. Placing PPR in the vacuole may benefit this complementary situation where one or the other phototrophic process is favored depending on the environmental conditions. And here, the presence of PPR in the vacuole may be especially beneficial for growth and survival as that organelle often acts as a storage site for cellular energy in the form of the phosphate anhydride bonds of polyphosphates. We hypothesize that this complementary behavior, along with the ability to store excess energy produced by PPR in the vacuole as high energy polyphosphates, represents an important survival strategy in the ocean, where light, iron levels, and temperature vary widely on a variety of spatial and temporal scales.

Assessment of the acceptance and implementation of child survival strategies among mothers in some selected communities in south-south Nigeria

Objective: The aim of this study was to assess the acceptance and implementation of child survival strategies through knowledge and practices among mothers in some selected communities in south-south Nigeria. Method: A descriptive survey method was used for the study and Rivers state which is in South-South Nigeria was randomly selected for the pilot study. The study was conducted between September 2021 and August 2023 and a sample size of Six Hundred respondents were randomly selected from the three senatorial districts of Rivers State. The instrument used for data collection was researchers’ constructed questionnaire with 17 items grouped into A and B. Section A contained information on demographic data of the respondents while section B focused on the Knowledge, practices and factors that influenced the acceptance and implementation of child survival strategies among mothers in some selected Local Government Areas in Rivers state. Data was analysed using descriptive statistics of percentages in frequency and tables. Results: The results of the findings showed a high level of knowledge (100%) and its components like skilled child birth attendance (92%), exclusive breastfeeding (83%), immunization (96%) and family planning (78.5%). Also majority of the mothers actually practiced the components of child survival strategies in this study, except family planning services (16%) despite being in the peak of their reproductive years and already having between four to six children. Other factors such as the attitude of the health workers and educational background of mothers were also identified as barriers to the practice of child survival strategies. Conclusion: It was therefore recommended amongst others that government at all levels should embark on aggressive health enlightenment campaign to further sustain these practices and also improve the acceptance and implementation of the practices of other child survival strategies yet to be accepted by mothers.